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rabbit polyclonal ca1  (Cusabio)


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    Cusabio rabbit polyclonal ca1
    A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , <t>CA1</t> , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.
    Rabbit Polyclonal Ca1, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal ca1/product/Cusabio
    Average 90 stars, based on 2 article reviews
    rabbit polyclonal ca1 - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids"

    Article Title: Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06680-z

    A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , CA1 , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.
    Figure Legend Snippet: A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , CA1 , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.

    Techniques Used: Light Microscopy, Cell Culture, Quantitative RT-PCR, Western Blot, Expressing, Marker, Incubation, Control, Immunofluorescence, Fluorescence



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    rabbit polyclonal ca1  (Cusabio)
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    Cusabio rabbit polyclonal ca1
    A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , <t>CA1</t> , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.
    Rabbit Polyclonal Ca1, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal ca1/product/Cusabio
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal ca1 - by Bioz Stars, 2026-03
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    anti ca1  (Cusabio)
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    Cusabio anti ca1
    A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , <t>CA1</t> , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.
    Anti Ca1, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ca1 polyclonal antibody  (Cusabio)
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    Cusabio anti ca1 polyclonal antibody
    Carbonic anhydrase 1 <t>(CA1)</t> expression and calcification in human aortic tissues. (A) CA1 protein expression in human atherosclerosis (AS) tissues and healthy aortic tissues using Western blot analysis. (B) Semiquantification of CA1 protein expression in human aortic tissues. (C) CA1 protein expression in human aortic tissues using immunohistochemistry (100X magnification). (D) Semiquantification of CA1-positive staining in human aortic tissues. (E) Calcification in human aortic tissues using von Kossa staining (100X magnification). (F) The expression levels of each CA member in human AS aortic tissues and healthy aortic tissues determined using real-time PCR. Data are shown as the means ± SEM. * P < 0.05, *** P < 0.001.
    Anti Ca1 Polyclonal Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ca1 polyclonal antibody/product/Cusabio
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , CA1 , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.

    Journal: Cell Death & Disease

    Article Title: Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids

    doi: 10.1038/s41419-024-06680-z

    Figure Lengend Snippet: A Light microscopy images of organoids (patient #110) cultured for 72 h in DIFF, BMP4, DBZ or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bar, 500 μm. B RT-qPCR analysis of the RNA levels of enterocytic ( KRT20 , CA1 , KLF4 and FABP2 ) and mucosecretory ( AGR2 , TFF2 and TFF3 ) genes in organoids from patients #47, #86, #110, #130 for 48 h and #47, #110, #130, #159 for 72 h cultured in DIFF (red), BMP4 (blue), DBZ (orange) or B + D (green) media in the absence (vehicle) or presence of calcitriol (100 nM). * P < 0.05, ** P < 0.01, *** P < 0.001. C RT-qPCR analysis of the RNA levels of stemness genes in organoids from the same patients and in the same conditions as in B. D Western blot analysis and quantification of the effect of calcitriol on the expression of enterocytic (CA1) and mucosecretory (TFF2) marker proteins in organoids from four patients incubated in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM) for 48 h. The stemness PTK7 protein was used as control of differentiation and GAPDH as loading control. * P < 0.05. E Immunofluorescence analysis of the expression of enterocytic and mucosecretory protein markers in organoids (patient #158) cultured for 48 h in PROL or B + D media in the absence (vehicle) or presence of calcitriol (100 nM). Scale bars, 30 µm. Box-plots show the quantification of fluorescence intensity (Log 2 MGV) (patients #92, #158 and #166; the number of organoids analyzed was 68 for KLF4, 104 for KRT19, 110 for AGR2 and 79 for TFF2). ** P < 0.01, *** P < 0.001. F Western blot analysis and quantification of the level of phospho(P)-SMAD1/5/8 in organoid cultures that were incubated for 24 h with calcitriol (100 nM) or vehicle before incubation in DIFF medium or BMP4 medium (50 ng/mL) during the indicated times. MGV: mean gray value.

    Article Snippet: Whole-cell extracts (15–30 μg or 80 μg the case of phospho-SMAD analyses) were separated by SDS-PAGE, transferred to PVDF membranes and incubated with the following primary antibodies: rabbit polyclonal-CA1 (CUSABIO, TX, USA, #CSBPA004364GA01HU), mouse monoclonal-GAPDH (Abcam, Cambridge, UK, #ab8245), rabbit monoclonal-PTK7 (Cell Signalling, MA, USA, #25618), rabbit polyclonal-SMAD1/5/8 (Santa Cruz, TX, USA, #sc6031-R), rabbit monclonal-Phospho-SMAD1/5/8 (Cell Signalling, #9511 S) and rabbit polyclonal-TFF2 (Proteintech, IL, USA, #13681-1-AP).

    Techniques: Light Microscopy, Cell Culture, Quantitative RT-PCR, Western Blot, Expressing, Marker, Incubation, Control, Immunofluorescence, Fluorescence

    Carbonic anhydrase 1 (CA1) expression and calcification in human aortic tissues. (A) CA1 protein expression in human atherosclerosis (AS) tissues and healthy aortic tissues using Western blot analysis. (B) Semiquantification of CA1 protein expression in human aortic tissues. (C) CA1 protein expression in human aortic tissues using immunohistochemistry (100X magnification). (D) Semiquantification of CA1-positive staining in human aortic tissues. (E) Calcification in human aortic tissues using von Kossa staining (100X magnification). (F) The expression levels of each CA member in human AS aortic tissues and healthy aortic tissues determined using real-time PCR. Data are shown as the means ± SEM. * P < 0.05, *** P < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis

    doi: 10.3389/fphar.2019.00766

    Figure Lengend Snippet: Carbonic anhydrase 1 (CA1) expression and calcification in human aortic tissues. (A) CA1 protein expression in human atherosclerosis (AS) tissues and healthy aortic tissues using Western blot analysis. (B) Semiquantification of CA1 protein expression in human aortic tissues. (C) CA1 protein expression in human aortic tissues using immunohistochemistry (100X magnification). (D) Semiquantification of CA1-positive staining in human aortic tissues. (E) Calcification in human aortic tissues using von Kossa staining (100X magnification). (F) The expression levels of each CA member in human AS aortic tissues and healthy aortic tissues determined using real-time PCR. Data are shown as the means ± SEM. * P < 0.05, *** P < 0.001.

    Article Snippet: The sections were incubated with an anti-CA1 polyclonal antibody (Cusabio, China) overnight at 4°C.

    Techniques: Expressing, Western Blot, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction

    Changes in histopathological structure, calcification, and CA1 expression in aortas from AS mice treated with MTZ. (A) CA1 protein expression in mouse model aortas using Western blot analysis. (B) Semiquantification of CA1 protein expression in mouse model aortas (ANOVA, P < 0.001). (C) CA1 transcription in aortas from the mice using real-time PCR (ANOVA, P < 0.001). (D) Sudan IV staining. (E) Semiquantification of atherosclerotic lesion area of AS mice (ANOVA, P < 0.001). (F) Oil Red O staining (40X magnification). (G) Semiquantification of atherosclerotic lesion area of the aortic root (ANOVA, P < 0.001). (H) Hematoxylin and eosin (HE) staining (40X magnification, the area indicated by the arrow is shown in the top right corner at 200X magnification). (I) Von Kossa staining (200X magnification). (J) Immunohistochemistry of CA1 (200X magnification). N = 5 for each group. Data are shown as the means ± SEM. ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis

    doi: 10.3389/fphar.2019.00766

    Figure Lengend Snippet: Changes in histopathological structure, calcification, and CA1 expression in aortas from AS mice treated with MTZ. (A) CA1 protein expression in mouse model aortas using Western blot analysis. (B) Semiquantification of CA1 protein expression in mouse model aortas (ANOVA, P < 0.001). (C) CA1 transcription in aortas from the mice using real-time PCR (ANOVA, P < 0.001). (D) Sudan IV staining. (E) Semiquantification of atherosclerotic lesion area of AS mice (ANOVA, P < 0.001). (F) Oil Red O staining (40X magnification). (G) Semiquantification of atherosclerotic lesion area of the aortic root (ANOVA, P < 0.001). (H) Hematoxylin and eosin (HE) staining (40X magnification, the area indicated by the arrow is shown in the top right corner at 200X magnification). (I) Von Kossa staining (200X magnification). (J) Immunohistochemistry of CA1 (200X magnification). N = 5 for each group. Data are shown as the means ± SEM. ** P < 0.01, *** P < 0.001.

    Article Snippet: The sections were incubated with an anti-CA1 polyclonal antibody (Cusabio, China) overnight at 4°C.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Staining, Immunohistochemistry

    Expression of CA1 protein and inflammatory cytokines in rat VSMCs with AZ treatment. (A) Western blot analysis of CA1 protein expression in rat VSMCs. (B) Semiquantification of CA1 protein expression (ANOVA, P < 0.001). (C) Cytokine levels in supernatant of cultured VSMCs by flow cytometry (ANOVA, P = 0.020, 0.003, 0.004, and <0.001, respectively). The results were obtained from three independent experiments. Data are shown as the means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis

    doi: 10.3389/fphar.2019.00766

    Figure Lengend Snippet: Expression of CA1 protein and inflammatory cytokines in rat VSMCs with AZ treatment. (A) Western blot analysis of CA1 protein expression in rat VSMCs. (B) Semiquantification of CA1 protein expression (ANOVA, P < 0.001). (C) Cytokine levels in supernatant of cultured VSMCs by flow cytometry (ANOVA, P = 0.020, 0.003, 0.004, and <0.001, respectively). The results were obtained from three independent experiments. Data are shown as the means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The sections were incubated with an anti-CA1 polyclonal antibody (Cusabio, China) overnight at 4°C.

    Techniques: Expressing, Western Blot, Cell Culture, Flow Cytometry

    Rat VSMC proliferation, migration, and apoptosis following anti-CA1 siRNA transfection. (A) Western blot analysis of CA1 protein expression in rat VSMCs transfected by anti-CA1 siRNA. (B) Semiquantification of CA1 protein expression relative to the internal control. (C) Rat VSMC proliferation using the Cell Counting Kit-8 (CCK-8) assay. (D) Transwell migration of rat VSMCs. (E) Statistical analysis of cell migration. (F) Representative image of rat VSMC apoptosis by flow cytometry. (G) Quantification of the apoptotic cell ratio. The results were obtained from three independent experiments. Data are shown as the means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis

    doi: 10.3389/fphar.2019.00766

    Figure Lengend Snippet: Rat VSMC proliferation, migration, and apoptosis following anti-CA1 siRNA transfection. (A) Western blot analysis of CA1 protein expression in rat VSMCs transfected by anti-CA1 siRNA. (B) Semiquantification of CA1 protein expression relative to the internal control. (C) Rat VSMC proliferation using the Cell Counting Kit-8 (CCK-8) assay. (D) Transwell migration of rat VSMCs. (E) Statistical analysis of cell migration. (F) Representative image of rat VSMC apoptosis by flow cytometry. (G) Quantification of the apoptotic cell ratio. The results were obtained from three independent experiments. Data are shown as the means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The sections were incubated with an anti-CA1 polyclonal antibody (Cusabio, China) overnight at 4°C.

    Techniques: Migration, Transfection, Western Blot, Expressing, Control, Cell Counting, CCK-8 Assay, Flow Cytometry

    CA1 expression, calcification, and inflammatory cytokine production in rat VSMCs with anti-CA1 siRNA transfection. (A) Western blot analysis of CA1 protein expression in rat VSMCs. (B) Semiquantification of CA1 protein expression (ANOVA, P < 0.001). (C) Calcification of rat VSMCs transfected with siRNA using AR-S staining. (D) Quantification of calcification using cetylpyridinium chloride (ANOVA, P < 0.001). Runx2 (E) , ALP (F) , and BMP2 (G) mRNA expression in rat VSMCs using real-time PCR (ANOVA, P < 0.001, < 0.001, and <0.001, respectively). (H) Cytokine levels in the supernatant of cultured VSMCs using flow cytometry (ANOVA, P < 0.001, < 0.001, < 0.001, and <0.001, respectively). The results were obtained from three independent experiments. Data are shown as the means ± SEM. ** P < 0.01 and *** P < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis

    doi: 10.3389/fphar.2019.00766

    Figure Lengend Snippet: CA1 expression, calcification, and inflammatory cytokine production in rat VSMCs with anti-CA1 siRNA transfection. (A) Western blot analysis of CA1 protein expression in rat VSMCs. (B) Semiquantification of CA1 protein expression (ANOVA, P < 0.001). (C) Calcification of rat VSMCs transfected with siRNA using AR-S staining. (D) Quantification of calcification using cetylpyridinium chloride (ANOVA, P < 0.001). Runx2 (E) , ALP (F) , and BMP2 (G) mRNA expression in rat VSMCs using real-time PCR (ANOVA, P < 0.001, < 0.001, and <0.001, respectively). (H) Cytokine levels in the supernatant of cultured VSMCs using flow cytometry (ANOVA, P < 0.001, < 0.001, < 0.001, and <0.001, respectively). The results were obtained from three independent experiments. Data are shown as the means ± SEM. ** P < 0.01 and *** P < 0.001.

    Article Snippet: The sections were incubated with an anti-CA1 polyclonal antibody (Cusabio, China) overnight at 4°C.

    Techniques: Expressing, Transfection, Western Blot, Staining, Real-time Polymerase Chain Reaction, Cell Culture, Flow Cytometry